pcr buffer Search Results


phusion high fidelity pcr master mix  (New England Biolabs)
99
New England Biolabs phusion high fidelity pcr master mix
Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+HF+Buffer/10__1038_slash_s41598___026___71175___0-91-8-13
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seqamp pcr buffer  (TaKaRa)
96
TaKaRa seqamp pcr buffer
Seqamp Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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10x qpcr buffer  (Jena Bioscience)
93
Jena Bioscience 10x qpcr buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
10x Qpcr Buffer, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/qPCR+Buffer/pmc11775725-131-38-46
Average 93 stars, based on 1 article reviews
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green gel loading buffer  (Jena Bioscience)
92
Jena Bioscience green gel loading buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Green Gel Loading Buffer, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Green+Gel+Loading+Buffer/pmc07936509__12951_2021_801_MOESM1_ESM-54-89-98
Average 92 stars, based on 1 article reviews
green gel loading buffer - by Bioz Stars, 2026-11
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pcr buffer  (agena bioscience)
96
agena bioscience pcr buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Pcr Buffer, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/PCR+Buffer/pmc06717854-139-15-21
Average 96 stars, based on 1 article reviews
pcr buffer - by Bioz Stars, 2026-11
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taq reaction buffer  (Jena Bioscience)
95
Jena Bioscience taq reaction buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Taq Reaction Buffer, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Taq+Polymerase+%2F+Labeling+Buffer/10__3957_slash_056__041__0105-37-5-8
Average 95 stars, based on 1 article reviews
taq reaction buffer - by Bioz Stars, 2026-11
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taq dna polymerase  (Valiant Co Ltd)
94
Valiant Co Ltd taq dna polymerase
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Taq Dna Polymerase, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Taq+DNA+polymerase/10__17930_slash_agr20241107-34-26-29
Average 94 stars, based on 1 article reviews
taq dna polymerase - by Bioz Stars, 2026-11
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5x mytaqtm buffer  (Meridian Bioscience)
94
Meridian Bioscience 5x mytaqtm buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
5x Mytaqtm Buffer, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Taq+PCR+Buffer%2C+5x/10__1016_slash_j__biocontrol__2022__104875-106-16-19
Average 94 stars, based on 1 article reviews
5x mytaqtm buffer - by Bioz Stars, 2026-11
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gel loading buffer  (Jena Bioscience)
90
Jena Bioscience gel loading buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Gel Loading Buffer, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Blue+Gel+Loading+Buffer/10__3923_slash_ajava__2017__205__211-53-13-19
Average 90 stars, based on 1 article reviews
gel loading buffer - by Bioz Stars, 2026-11
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dye  (Jena Bioscience)
90
Jena Bioscience dye
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Dye, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Blue+Gel+Loading+Buffer+with+DNA+Stain/pm29340452-43-7-8
Average 90 stars, based on 1 article reviews
dye - by Bioz Stars, 2026-11
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pcr buffer  (Genesee Scientific)
93
Genesee Scientific pcr buffer
The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time <t>quantitative</t> <t>PCR</t> and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.
Pcr Buffer, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/PCR+Buffer+I%2C+Mg+Free/pmc07593134-83-10-31
Average 93 stars, based on 1 article reviews
pcr buffer - by Bioz Stars, 2026-11
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Image Search Results


The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time quantitative PCR and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.

Journal: Experimental and Therapeutic Medicine

Article Title: Long non‑coding RNA signatures in breast cancer: Properties as biomarkers?

doi: 10.3892/etm.2025.12804

Figure Lengend Snippet: The influence of treatment on lncRNA expression levels of the different BC cell lines. Expression profiles of selected lnc-RNAs in BT-474, MCF-7, T-47D, SKBR-3, BT-20 and MDA-MB-231cells under control conditions and after different breast cancer treatments. Lnc-RNA-expressions were determined in triplicates by real-time quantitative PCR and calculated using ∆Ct method based on reference value (geometric mean of housekeeping genes Alas1 and TOP2α) to determine expression of single lncRNAs. After calculating the expression level. The data were visualized in a heatmap according to the color key. It represents the fold change of the ∆∆Ct values compared to the same cell line untreated. lncRNA, long non-coding RNA; CCAT2, colon cancer associated transcript 2 RNA; CYTOR, cytoskeleton regulator RNA; HOTAIR, HOX transcript antisense RNA; MALAT-1, metastasis associated lung adenocarcinoma transcript 1; NKILA, NF-ĸB interacting long non-coding RNA TERC, telomerase RNA component; WSPAR, WNT signaling pathway activating non-coding RNA.

Article Snippet: The relative expression levels of specific mRNAs and lncRNAs were assessed by qPCR by using a SYBR Green assay in duplicate. cDNA (1 μl) was mixed with 9 μl Master Mix containing 6.45 μl nuclease-free water, 1 μl 10X qPCR buffer, 0.5 μl 5 mM dNTPs (Jena Bioscience), 0.5 μl specific qPCR primer (Biomers), 0.5 μl SYBR Green 1 μM (Jena Bioscience) and 0.25 U Hot Start Taq-Polymerase (Jena Bioscience).

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction